4 edition of Enzymological and physiological studies of bacteriophage T4 induced RNA ligase found in the catalog.
Enzymological and physiological studies of bacteriophage T4 induced RNA ligase
Thomas J. Snopek
Written in English
|LC Classifications||Microfilm 69125|
|The Physical Object|
|Pagination||vi, 90 p.|
|Number of Pages||90|
|LC Control Number||94895174|
Other examples of RNA ligases include the L1 ligase (Robertson and Ellington), the R3C ligase (Joyce), the DSL ligase (Inoue). The most active ligase known to date is the Class I ligase, isolated from random sequence (work of David Bartel, while in the Ligase ribozymes may have been part of such a pre-biotic RNA world. In order to copy RNA, fragments or monomers (individual. The bacteriophage T4 encodes 10 proteins, known collectively as the replisome, that are responsible for the replication of the phage genome. The replisomal proteins can be subdivided into three activities; the replicase, responsible for duplicating DNA, the primosomal proteins, responsible for unwinding and Okazaki fragment initiation, and the Okazaki repair proteins.
Mutants of bacteriophage T4 deficient in the ability to induce nuclear disruption. II. Physiological state of the host nucleoid in infected cells. J Mol Biol. Nov 15; 89 (4)– Stevens A. New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4. procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identiﬁed bacteriophage T4-dependent modiﬁcations of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identiﬁcation of bacteriophage-dependent alterations of the host synthesis machinery.
T4 phage lysis genes were cloned into pET vector plasmid. Expression of the target gene encoded in this vector is under control of bacteriophage T7 transcription and translation signals: expression is induced by providing a source of T7 RNA polymerase in the host cell of E. coli BL21(DE3)pLysS. BL21(DE3)pLysS contains a plasmid carrying a T7 lysozyme gene which is a natural inhibitor of T7 RNA. Abstract. The total map length of the bacteriophage T4 genome has been determined by electron microscopy of T4 DNA molecules to be × 10 5 nucleotide pairs (Kim and Davidson, ). In Figure 1, each small division on the inner circle indicates 10 3 nucleotide pairs. The zero point has been placed arbitrarily at a genetically and physically well-defined locus, the divide between the rIIA.
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Bi-Level Integrated System Synthesis (BLISS)
RNA ligase and tail fiber attachment activities, normally induced following bacteriophage T4 infection of Escherichia coli, are not induced when gene 63 amber mutants of T4 infect nonpermissive host cells. Both activities are induced when these mutants infect permissive hosts, or when revertants of these mutants infect nonpermissive by: T4-induced polynucleotide kinase, RNA ligase, and a tRNA-specific endonuclease were previously implicated in host tRNA breakage and reunion.
to examine this hypothesis we followed the fate of host tRNAs in T4-infected Escherichia coli CTr5x, a strain restricting phage mutants lacking polynucleotide kinase (pnk −) or RNA ligase (rli −).Two host tRNA species which were cleaved in the Cited by: 9.
applications, including its DNA and RNA ligase, polynucle-otide kinase, and DNA polymerase. Many would argue that to know T4 is to know the foundations of molecular biology and the essential paradigms of genetics and gene expression. There was a price to pay for all of the beneﬁts provided by this highly tractable genetic by: T4 RNase H removes the pentamer RNA primers from DNA chains initiated by the T4 primase-helicase (gene products 61 and 41).
Addition of T4 RNase H and T4 DNA ligase leads to extensive joining of discontinuous lagging strand fragments in the T4 DNA replication system in vitro. Bacteriophage. All of the bacteriophage used in this study originated from the Doermann strain of T4 (T4D). The rI mutant, r48, has been described by Doermann and Hill ().The ts endolysin mutant eC was obtained from George am mutants employed in this study were isolated as described below.
Bacteriophage stocks were prepared on strain CRCited by: 6. Since their discovery in the early twentieth century, bacteriophages (phages) have played a central role in understanding many key principles in molecular biology. In particular, they were essential.
The RNA is then 5'-adenylated using ATP and T4 RNA ligase, in an interrupted version of the natural adenylation-ligation mechanism by which T4 RNA ligase joins two RNA substrates.
  One of the most widely used ligases in vitro is bacteriophage T4 DNA ligase, which is a single kDa polypeptide that requires ATP as an energy source. 8 T4 DNA ligase typically joins. EPB1 EPA EPA EPB1 EP B1 EP B1 EP B1 EP A EP A EP A EP B1 EP B1 EP B1 Authority. The Escherichia coli lit gene product is required for the multiplication of bacteriophage T4 at temperatures below 34 degrees C.
Genetic and Physiological studies of the role of the RNA ligase. Thus, the T4‐induced anticodon nuclease cleaves lysine tRNA 5′ to the wobble position, yielding 2′:3′‐P greater than and 5′‐OH termini. Polynucleotide kinase converts them into a 3′‐OH and 5′ P pair joined in turn by RNA ligase. SUMMARY Phage T4 has provided countless contributions to the paradigms of genetics and biochemistry.
Its complete genome sequence ofbp encodes about gene products. T4 biology and its genomic sequence provide the best-understood model for modern functional genomics and proteomics.
Variations on gene expression, including overlapping genes, internal translation. Abstract. By far the best-known representative of the large virulent bacteriophages is coliphage T4, which has been an object of intense biochemical, genetic, and morphological investigation since its original description some three decades ago (Demerec and Fano, ).
The σ factor is a functionally obligatory subunit of the bacterial transcription machinery, the RNA polymerase. Bacteriophage-encoded small proteins that either modulate or inhibit the bacterial.
RNA ssDNA dsDNA ss MS2 ds φ6 φψ Adapted from an illustration by New England BioLabs, Inc. Figure Electron micrograph of bacteriophage P1. Photo courtesy of Michel Wurtz and the Biocenter at the University of Basel.
Figure Transducing particle (contains gaps by DNA ligase cos att cos. T4 Bacteriophage – History, Structure, Graphical Life Cycle, Phage Therapy. T4 Bacteriophage or simply phages are the type of viruses which attacks bacteria. The bacteriophages are among the most complex viruses. They have a complex shape like a tadpole i.e., bacteriophage T4, which attacks Escherichia coli (bacterium).
Bacteriophage T4 and its host bacterium, Escherichia coli, can be considered among the earliest model organisms – biological systems that attract large numbers of investigators who, because of technical or conceptual advantages of the system, use the system to investigate processes and mechanisms of general significance in ing in the mid‐twentieth century, studies focused on.
Bacteriophage T4 codes for RNA ligase 1 and RNA ligase 2 ligating ssRNA and dsRNA molecules, respectively. In vivo RNA ligase 1 repairs a break in the anticodon loop of the host tRNA Lys introduced by an E. coli encoded nuclease, whose expression is induced by the phage infection.
The in vivo significance of RNA ligase 2 for the T4 biology is. Figure 1 Structure of T4 bacteriophage: COMPOSITION AND STRUCTURE OF BACTERIOPHAGE. Composition. Although different bacteriophages may contain different materials they all contain nucleic acid and protein.
Depending upon the phage, the nucleic acid can be either DNA or RNA but not both and it can exist in various forms. Fig. Bacteriophage T4 and its long tail fibers. (A and B) Schematic representations of bacteriophage T4 attached to a bacterial membrane before (Left) and after (Right) contraction of the outer tail tip domain of gp37 is boxed.
(C) Schematic representation of the bacteriophage T4 long tail s P1–5 correspond to gp34; the kneecap domain (K-C) is formed by gp35. Escherichia virus T4 is a species of bacteriophages that infect Escherichia coli bacteria. It is a double-stranded DNA virus in the subfamily Tevenvirinae from the family Myoviridae.T4 is capable of undergoing only a lytic lifecycle and not the lysogenic species was formerly named T-even bacteriophage, a name which also encompasses, among other strains (or isolates.Silber R, Malathi VG, Hurwitz J.
Purification and properties of bacteriophage T4-induced RNA ligase. Proc Natl Acad Sci U S A. Oct; 69 (10)– [ PMC free article ] [ PubMed ].We have tested for the presence of the receptor for the Escherichia coli phage T4 in different isolates of the plant pathogenic enterobacteria Erwinia carotora subsp.
carotovora and subsp. atroseptica. Several of the isolates appeared to contain a functional T4 receptor as shown by phage adsorption and phage-induced lysis of the bacteria. Two of the isolates could even sustain lytic growth of T4.